Sample
population and size:
The
blood of some apparently healthy volunteers from Madonna University Teaching
Hospital (MUTH) will be used for the study. The ratio of male to female would
not be significant.
Ethical
Clearance:
The
Ethical Committee consent will be sought and also consent from candidate will
be sought.
Other materials
and reagents:
Ten
mls syringe, cotton wool, tourniquet, 70% alcohol, heparin anticoagulant (3iu
per bottle), ethylenediaminetetra acetic acid
(EDTA) anticoagulant (3.5mg per bottle), sodium citrate container
(6.5lmg per container), fluoride oxalate (49mg per container), cover slips,
Neubeur counting chamber, lml pipette, automatic micropipette, plain tubes,
racks and microscope.
METHODS
Sampling and
sample size:
The
samples will be collected by just explained to the students what their blood is
going to be used for hence questionnaires are not necessary. Eight Milliliters
(8mls) of venous blood will be collected from each subject by venous puncture
using aseptic methods.
Procedure:
The
left median antecubital will be used for all subjects to maintain uniformly.
The blood samples will be immediately placed in different sterile container containing
specific anticoagulants to be used for the study. Each anticoagulant container
will contain 2mls of blood, four (4) types of anticoagulants will be used for
the study. The final concentration of each of the four anticoagulants will be
2mls of blood to – dispotassium ethlenediaminetetra – acetic acid KI2EDTA
3.5mg, heparin 3iu, fluoride oxalate 49mg, and sodium citrate (NaC)
6.51mg. the anticoagulatnted blood sample will be stored at 4oC from
where samples will be taken on a daily basis for four (4) consecutive days for
platelet count (Normal range 100-400x109/Liter) Dacie and Lewis 1994).
Method of Platelet
Count:
The
method to be adopted will be that contained in Chessobrough 2006. The platelet
count will be done using the Improved Neubeur Counting Chamber and cover slips.
The blood will be diluted with platelets diluting fluid (1% ammonium oxalate)
in the ratio of 1:20 using the improved Neubeur Counting Chamber the platelets
per liter of blood will be calculated.
Principle of
test:
Blood
is diluted I in 20 in a filtered solution of ammonium oxalate rreagent which
lyzes the red cells. This method of platelet count is that of Cheesbrough 2006.
How result
values will be analyzed;
Three
readings will be taken in each case; the mean values and the standard deviation
will be calculated and recorded. The values obtained will be subjected to
“student test” using 150x109/L as the standard and Day as the
control or base line. P. value (degree of freedom expressed in percentage) of
<0.05 will be taken as significant statistically.