MATERIALS: Traditionally fermented product of African oil bean
(Ugba), locust bean (iru) mesquite seed (Okpei) and castor bean (Ogiri) will be
bought from Abakpa market Abakaliki in Ebonyi state Nigeria.
Isolation of microorganisms
Fermented
samples (Ugba, Ogiri, Iru, Okpei) were taken aseptically from traditionally
fermented beans. One gram of the sample will be thoroughly mashed with
laboratory pestle and motar and mixed with 9ml of normal saline water as a
diluents in a McCarteny bottle and the content was thoroughly shaken.
Subsequent serial dilution
(10-2, 10-3, 10-4,
10-5, 10-6) were made from this solution by adding
serially 1ml of solution from preceding concentration of 9ml of the diluments,
using sterile syringe. The nutrient agar will be prepared by adding 11ml of
distilled water to 2.8g of agar in a conical flask. Unto a Petri-dish
containing 0.1ml of the inoculums, 1 to
15ml of the sterile warm (45oc) nutrient agar will be poured. The plated will
be labeled appropriately, inverted and incubated at 30oc for 24hr in a
Gallenkamp incubator. 
MATERIALS AND METHODS
The traditional fermented food
condiments Mesquite
seed(okpei), fermented oil bean (ogiri ugba), fermented melon seed (ogiri
egusi), fermented soy bean (dawadawa) Uziza,and Uda. The samples were purchase
at Abakpa main market, Abakaliki, Ebonyi
State.
METHODS - SAMLPES PREPARATION
The samples were put in the crucible and
then transfer to the furnace for 2-3hrs at the temperature of 5500c.
ZINC (A.O.A.C.2001)
Measure 20ml of the sample into the
measuring cylinder, add 2ml of buffer 10solution. Add 3drops of ERDTI BLACK T,
titrate with 0.01ml of EDTD until it turns to blue black then take the reading
of the initial and final reading.
IRON (A.O.A.C.2001)
Measure 5ml of digested sample and pour
into 5ml of volumetric flask.
Measure 5ml of sodium acetate 17% or
buffer 4solution.
Measure 0.5ml of hydroxylamine hydrochloride
10%.
Measure 5ml of phenoltroline 0.25%, and
then make it up to 50ml in the volumetric flask using distilled water.
Allow to develop for about 20-30mins before absorbance
reading using spectrometer.
IONDINE
(A.O.A.C.2001)
Measure 20ml of the digested sample,
shake using electric shaker. Add 1ml of 2NH2SO4, 5ml of 10% KI. Stop the flask
and keep in the dark for 10mins. Bring it out and titrate with 0.002N Na2SO4
until the solution becomes purple pale yellow. Continue until the solution
becomes colorless, check the burette reading.
SODIUM (A.O.A.C.1984)
A stock solution containing 100mg/ml of
solution ion was prepared by dissolving 2.542g of oven dried Nacl in de-ionized
water and subsequently diluted to mark, from this solution standard, solution
with conc. 0.0,0.1, 0.2 and 0.3ppm are prepared.
POTASSIUM
(A.O.A.C1984)
A stock solution containing 100mg/ml of
K+ ions is prepared by dissolving 1.907g of kcl in water.
The solution will be made to mark with distilled water, from this stock
solution standard, solution conc 0.0, 2.0, 4.0 and 6.0ppm will be prepared. A
stock solution of 1% lithium-chloride will be prepared and add to the potassium
and sodium standard solutions such that the concentration in the final solution
is 0.1%
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