CONCLUSION
Blood film assessment is the basic assessment to
identify a good hematological practice. Blood film making is a daily routine in
all hematology laboratories and should be carefully handled. A well made blood
film gives accuracy to any analysis its being used for. So also is a
well-stained blood film. Blood films are products of the laboratory and should
therefore be subject to checks on their consistency and quality in terms of
minimum requirements for acceptability.
It is the responsibility of the
laboratory management to put such checking procedures in place. In an entirely
manual environment, sufficient training and—when necessary—remedial training
should be offered to the laboratory staff to ensure that blood films of
sufficient and consistent quality are being prepared. In automated and
semi-automated environments, staff should be properly trained in instrument
use, and records of instrument maintenance should be kept.
The choice of stain
and stain preparation also must be carefully analyzed to acquire a bright and
well stained films for proper differentiation and identification. Over-staining
in hematology is advice to be avoided because if the cells are over stained,
microscopy will be altered. Accurate timing should be observed while staining,
as stated in this seminar work. Conclusively, it is worthy of Note that a good
blood film makes a good hematologist because blood film making is one of the
basis in hematology practices.
RECOMMENDATION
The laboratory
is recommended to participate in (external) proficiency testing programs,
although such programs mostly test the laboratory’s ability to properly stain a
blood film and assess the morphological skills of the staff. In external
proficiency testing programs, logistics and the aging of blood specimens usually
prohibit the blood film preparation itself, especially when blood specimens are
mailed out to the individual institutions.
APPENDIX
· EDTA means
ethylene diamine tetra acetic acid. this can also be taken for EDETATE which is
a salt of EDTA.
· WHO means world
health organization. This arm pose as a standard of ensuring better health
practice in the world.
· HEPA means
high-efficiency particulate air.
· CLL means
chronic lymphocytic leukaemia. This shows increase in the number of lymphocytes
in the blood and bone marrow.
· Turks solution
is a solution containing Acetic acid 2%v/v and a tinge of gentian violet. It is
used for white cell count.
· RBC means red
blood cell
· WBC means white
blood cell
· Neubauer
counting chamber is a counting chamber that consists of a thick rectangular
glass slide with an ‘H’ shaped trough, forming two counting areas.
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