THROMBOCYTES AND PLATELET

These are membranes bound cell fragments, having no nucleus they are between 1-2um in diameter and are about 1/10th to 1/20th abundant to white blood cells. The production of platelets results from the fragmentation of large cells called megakaryocytes which are cells derived from stem cells in the bone marrow. They are produced ata rate of 200billon/day. The production is regulated by a hormone known as thrombopoiesis. The circulating life is 8-10 days.
The stick surface of the cells allows them to accumulate at the site of broken blood vessels to form a clot. The normal of this cell is between 150-450 x 10/L Blood clotting is the chief function of platelets and this can be achieved in 3 states are:
1.      Vascular spasm or vasoconstrictor.
2.      Formation of platelet plug.
3.      Blood clot formation.

Vascular spasm or Vasoconstriction:
In a normal individual, immediately after a blood vessel has been cut open and endothelial tissues are damaged, vasoconstriction occur thus slowing blood flow to the area. The vascular spasm usually lasts for about 30 minutes, long enough for the next stage of homeostasis to take place.

Formation of a plan platelet plug:
within 20seconds of an injury, coaglation is initiated. Contray to the popular belief that clotting of a cut on the skin is not initiated by air or drying out, but by platelet adhering to and activated by collagen in the blood vessels endothelium. The activated platelet then release the content of hteir granules, which contain a variety of substances that stimulate futher platelet activation and enhace the homoeostatic process. This process involves lining up of thre broken blood vessels and endothelial cells, revealing proteins in the vessel wasll, platelets swell, grow spiky extensions and starts clumping together. This results in a platelet plug that seals the injured area. if the injury is small, a platelet plug may be able to form and close within seconds. Platelets contain secretory granules and when they sick to the proteins in the vessel walls they degranulate thus releasing their products which include ADP (adenosine diphosphate), serotonin and thromboxane A2.

Blood clot formation:
here the blood changes from a liquid to a gel, at least twelve substances called clotting factors takes place in a series of chemical reactions that eventually creates a mesh of protein fibers within the blood. This can be achieved by the sole function of the clotting factors; these are produced and deposited in the blood by the liver.
Normal range:
Adult: 150-450 x 109/L (Cheesbrough, 2006).
200-400 x 103/ul (Ezeilo, 2002)
2.7 erythrocyte sedimentation rate (ESR)
this test measure how quickly erythrocytes settles in at the bottom of a test tube for an hour, when there is occurrence of inflammation in the body, certain proteins cause red blood cells to stick together and fall more quickly than normal to the bottom of the tube. This is a non-specific test which is raised in a wide range of infectious, inflammatory, degenerative and malignant conditions associated with changes in plasma proteins, particularly increase in immunoglobulins, C-reactive proteins and fibrinogen. Moderately raised sedimenation rates can sometimes be seen in healthy people (Cheesbrough, 2006). The test is done using the Westergreen method which is gotten in millimeters per hour. ESR can be increased by any cause or focus of inflammation such as in pregnancy or rheumatoid arthritis, it can be decreased in sickle cell anaemia, polythaemia. ESR values are higher in women as do the elderly (Brigden; 1998).
Normal value for male: 10mm/hour (Cheesbrough, 2006).

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