Laboratory diagnosis and Treatment of Tuberculosis

DIAGNOSIS OF TUBERCULOSIS
Sputum microscopy: Sputum microscopy is the most efficient way of identification of tuberculosis infection. In areas with high prevalence of tuberculosis, microscopic examination of smear should be initial test.

Culture: Mycobacterium culture is useful for the definitive confirmation of tuberculosis and in drug sensitivity determination. Its role becomes important with declining prevalence of tuberculosis in a community. The Lowenstein Jensen medium is the commonest culture medium to use and is most often combined with sputum microscopy (Booker, 1996).

TREATMENT OF TUBERCULOSIS


Treatment for TB uses antibiotics to kill the bacteria. The two most commonly used are Rifampicin and Isoniazid. However, instead of the short course of antibiotics typically used to cure other bacterial infection, TB requires much longer periods of treatment around (6-12 months) to entirely eliminate mycobacteria from the body. (Centers for disease control).
The aims of TB treatment include:
1.    To decrease mortality, long term morbidity and transmission
2.    To affect a permanent cure, prevent relapse and decrease transmission.
3.    To achieve the above with minimum side effect.
4.    To minimize the development of drug resistance.
Drug used in anti- tuberculosis treatment:
First line drugs include                          
·         Isoniazid (H)
·         Rifampicin (R)
·         Pyrazinamide (Z)
·         Ethambutol (E)
·         Streptomycin (S)
·         Thiacetazone (T)
Second line drugs
          These are those drugs which should be reserved for use in drug- resistant cases only. e.g  Ethionamide and prothinanamide .
 Treatment of tuberculosis is in two phases:-  
Intensive and Continuation phases.
Intenssive phase entails,
1.    Rapid killing of bacteria, resulting in
2.    A state of non- infectiousness within 2 weeks.
Multiple drugs are combined in intensive phase.
Continuation phase lasts for 6 months using fewer drugs for longer time to eliminate the remaining bacilli.
F. Directly observed treatment short course (DOTS)
          DOTS is a strategy to ensure cure by providing the most effective medicine and confirming that it is taken. It was adopted in 1993  by the WHO and is the only strategy which  has been documented to be effective world wide on a program basis. In Dots during the intensive phase of treatment a health worker or other trained person watches as the patient swallows the drug in his presence. During continuation phase the patient is issued medicine for one week in a multi blister combipack of which the first dose is swallowed by the patient in the presence of health worker or trained person. The consumption of medicine in the continuation phase is also checked by return of empty multiblister combipack when the patient comes to collect medicine for the next week.
          The whole idea is to ensure compliance by monitoring the patient. Treatment of TB lasts long and the early arrest of the bacilli in the first 2 months is important to avoid drug resistance (K.Parks. pg 146).

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