PROXIMATE COMPOSITION OF SOME LEGUME SEED SAMPLES MOISTURE


The moisture content of the five legume flour samples (Breadfruit, Groundnut, Soybean, Bambara-groundnut and Cowpea). Analyzed was found to be 10.87, 7.66, 7.85, 10.36, 10.44 respectively with breadfruit having the highest value. The samples showed significant difference at (P<0.05). The groundnut flour having lower moisture content can be stored for a longer period than the other legume.
            High water moisture content in flours, predispose them to bacterial and fungal attack. As a result of the value of the moisture content of soybean flour not being so high, it can also be stored for a long period of time. The values for groundnut and soybean flour fell within range (5-8%) given by Yagodin (1984) for legume.

PROTEIN
            The protein content in the legume was 17.45, 25.35, 39.86, 16.64 and 23.36 for breadfruit, groundnut, soybean, bambara-groundnut and cowpea respectively with soybean having the highest value.
            At P<0.05, there is significant difference between the legume samples.
            This range of values falls within the range given by Tobin and Carpenter (1978) which is 15-30%. The protein content of the legume was than those reported by Martin- Cabrejas et al. (1994). Soybean are rich source of protein. Dietary protein are needed for the synthesis of new cell, repair of worn out tissues, enzymes, hormones, antibiotics and other substance required for healthy functioning development of the body and its protection (cheese Brough, 1987) and for the treatment of protein energy malnutrition (Omoruiyi et al; 1994).

ASH
            The ash content of the samples breadfruit (1.45) groundnut (4.87) soybean (3.56). Bambara groundnut (2.95) and cowpea (3.78) was observed, to be a little higher compared to results of the above workers it is however in agreement with the observations of Esenwah and Ikere Bomeh (2008) and Omafuvbe et al. (2004) who report higher values. The bio-chemical analysis of seeds of the legume showed that the ash contents ranged from 1.45 to 4.87g /100g which were found to be low as compared to those reported by Udayasekhara (1995) but comparable to those reported by Fasoyiro et al. (2006). Ash content of groundnut and cowpea was as high as 4.87% and 3.78%, respectively and that of other legumes ranged between 1% and 3.80%, there were significant difference at P<.0.05. this range of values obtained falls within the range fiven by Ologhobo and Fetuga (1988) and Yeshajahu (1991) as 4.1 – 4.77 and 2.8 – 4% respectively. With these ranges of values, it means that small amount of inorganic compounds are present in the legume samples.

STARCH AND REDUCING SUGARS
            The starch and reducing sugars contents obtained in this study was low while the dry matter was higher than those reported. These differences could be attributed to environmental factors, as well as the cultivars of the different legume seeds and the experimental procedure used. Reducing sugar levels has been reported to decrease  with processing (Omafuvbe, 2004). The reducing sugar is higher in groundnut and the starch content is higher in breadfruit seeds. This variation is attributed to the high energy content of the legume (breadfruit and groundnut).

FIBER
The fibre content of the legume samples analyzed was found to be 2.44, 3.84, 3.87, 4.93 and 4.02 for breadfruit, groundnut, soybean, bamabara groundnut and cowpea respectively, with Bambara groundnut having the highest value.   The legume samples showed significant difference at p<0.05. The fibre content in relation to the diet is adequate, exerting a major influence on the metabolism of the gastrointestinal tract (GIT) and constituents its deficiency linked to appendicitis, biventricular disease and hemorroids (Gibney, 1989). Fibric also sows down the release of glucose into the blood and decrease intercolonic pressure, hence, reduces the risk of colon cancer (Gibney, 1989). This range of values obtained falls below the range reported by Gibney (1989) which is 13-19%.

FAT
The fat content obtained from the analysis of the regume samples was 7.65, 47.57, 20.42, 5.57 and 2.35 for breadfruit, groundnut, soybean, Bambara-groundnut and cowpea, respectively with the groundnut  flour sample having the highest value, cowpeas have been shown to be low in fat content (Davidson et al., 1975). At p<0.05, there was significant different between the legume flour samples, fat provide strong energy and transports fat soluble vitamins like vitamin A, D, E and K (Ologhobo, 1988).
            The range of value for cowpea flour, obtained falls within the range of 2.01-2.88% reported by Ologhobo and Fetuga (188). The value for the other legume flour samples are higher than these   ranges.

Table 2: Dietary fiber in some legume seeds.
Legumes
SDF
IDF
TDF
IDF/SDF RATIO
Breadfruit
1.66d
40.66d
42.02c
24.32bc
Groundnut
1.87c
39. 87e
41.73e
21. 32a
Soybean
2.06a
40.23c
42.28a
19.53b
Bambara groundnut
1.95b
69.98d
41.93d
35.89bc
Cowpea.
2.04a
40.16d
42.20b
19.69bc

The result on the dietary fibre content of the five (5) grain legumes are presented in table 2. It is evident that the total detry fibre (TDF) content of legume seeds varied form 41.73 to 42.28%. soybean was the richest source of fibre, followed by cowpea, bread fruit, Bambara groundnut, and groundnut. Soybean seeds also contained the highest amount of soluble fibre (2.06) among the other legumes. The legumes were not good sources of soluble fibre as they do  not contained about 5% SDF commonly found in legume seeds. The TDF content of cowpea as found in this study do not agrees to that   and Swanson (1989) who reported that cowpea contained 14 to 19% TDF, However, Sharma (1986) found 17 to 23.4% TDF in cowpea.
            The water insoluble to soluble fibre ratios (Table 1) indicates that all the legumes contained higher proportion of insoluble fibre than the soluble fibre. This ratio was higher in both Bambara groundnut and breadfruit, indicated that the fibre of these legumes was mainly composed of cellulose, constituents of insoluble fibre. The soluble fibre (pectin and few other non-starch polysccharides). Being viscous gels liner along the walls of the intestine and thus reduces glucose and cholesterol absorption into the blood stream (Anderson et al., 1984 and Toppy et al, 1986). This helps in dipping low blood sugar and cholesterol levels which is most beneficial for diabetic and heart patents (Kritchevsky 1986). Since legumes are better source of soluble fibre than cereals (Hughes and Swanson, 1989). They are particularly recommended in the diets of both diabectic and heart patient.
            Dietary guidelines recommend a minimum daily intake of DF of 25g (equivalent to  12.5g DF per 1000 calories consumed). (Martett et al, 2002, USDA, 2000); which is considerably higher than the estimate instates in western countries (sungsocho and Dreher, 2001). Public health organization (ADA, 1997). Recommended that adult should take 25 to 35g dietary fibre per day with adequate fluid intake. Therefore there is a need to increase fibre intake, which has prompted the consumption dietary supplements or fibre-enriched food products. Dietary fibre components like pectins, gum, cellulose and others have been used as functional ingredients by the food industry, with an extensive market of food by-products as dietary fibre sources.
            Some of the health benefits associate with a high fibre diet may came the vitamin, minerals or non-nutritive photochemical present in high fibre foods rather than from the fibre itself. Some health effects associated with fibre might also be due to the substitution of high fibre foods for less healthful foods, to the relatively low fat and calorie levels in most high-firbre diets, or to the correction between high-fibre diets and other healthful life style choices (such as regular exercise). In addition comparisons made between population groups (such as the observations made by Dr. Burkitt) may not be directly applicable to individuals (Just because societies that eat high-fibre diets have a low frequency of constipation, for example, it does not necessarily follow than an increase in fibre intake would relieve constipation in a specific western individual ). The best accepted beneficial effect of dietary fibre is relief of constipation (Marlett, 1992). Physicians frequently recommend a trial of high-fibre diet as a  first step in the treatment of constipation. I adults (Ada, 1993; Gray, 1995) and as treatment for irritable bowel syndrome (A functional gastrointestinal disorder that may involve constipation and/or diarrhea) (Bennett and Cerda, 1996).
            Dietary fibre, in conjunction with other therapies, is also used to treat chronic constipation in childhood (Mcclung, 1995, Hillemeier, 1995).
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