WHAT IS THE MAIN AIM OF THE PRACTICAL
The major
objective of the practical is to
(1) Impact the
knowledge of artificial induced breeding to learners and beginners.
(2) For first
time learners to know that is better and more reliable because
(i) better rate
of fertilization and hatching will be achieved.
(ii) protection
against enemies and unfavorable environmental condition.
(iii) better
condition for growth and survival.
PROCEDURE INVOLVED IN FISH BREEDING
(1) Selection of
brood stock from fish pond.
(2) Inducing
final maturation and ovulation with hormone treatment.
(3) Procurement
of ripe eggs by stripping.
(4) Procurement
of milt by dissection of male donor.
(5) Artificial
fertilization.
(6) Rearing of
larvae or fry.
SELECTION OF BROODSTOCK FROM FISH POND
The brood stock
was selected and kept in plastic tank filled with water. The female breeder
showed the maturity by bringing out a brighter colour and the abdomen, was
swollen, lighter brownish in colour, when applied pressure on the abdomen, some
eggs quickly come out with light green and a dot in the middle. In the male
breeder, the genital papillae was elongated dark and slightly swollen.
INDUCED FINAL MATURATION AND OVULATION WITH
HORMONE
On the next day,
the breeder was brought out of tank and placed on top of the table by 8:05am to
weigh by the use of weighing scale.
·
The male brood stock weighed 2.0kg
·
The female brood stock weighed 3.0kg.
·
The temperature of the environment is 28 degree
cen.
·
The temperature of water 27 degree cen.
·
The female breeder was injected with ovaprim (1.5mm)
dose by 8:12am intra muscularly at angle 45 degree above the lateral line and
above the dorsal fin.
·
The latency period was 8 hours (time of
injection and ovulation).
PROCUREMENT OF MILT BY DISSECTION OF A MALE
DONOR
The male was
dissected and to obtain the tastes which are located at the dorsal part of
abdominal cavity of the male. The milt was obtained from the tastes by cutting
little incisions in the external part of the lobules. The milt was preserved temporally
in glass cup and covered.
PROCUREMENT OF THE RIP EGG BY STRIPPING
The ovulated
eggs in the female breeder was obtained when the eggs ripped.
The ovulation
period reached by 5:15pm. The belly was thick and soft.
Two person held
the fish with wet towel to avoid injury to the fish.
The ventral side
of the fish was dried up with toilet tissue and gently pressed towards the
genital papillae to extrude the eggs.
ARTIFICIAL FERTILIZATION
We used dry
method of fertilization by adding the milt on the stripped eggs and few drops
saline solution to ensure proper mixing of the sexual product through gentle
shaking of the bowel and stirred
.
INCUBATION AND HATCHING OF FRY
We washed the
plastic bowel and filled close to the brim with clean water. The fertilized
eggs were spread homogenously in a single layer on a kakaban (mosquito netting
material placed on top of the water in bowel.
LARVAL REARING
We separated the
larvae from egg shell and un-hatched eggs. The kakaban was removed which helped
in separating the larvae from the shell. The remaining un-hatched eggs were
removed by siphoning.
NURSING (FEEDING) FRY
Feeding started
after the third day of hatching, we introduced Artemia naupilli. We fed with
Artemia for seven days before changing to 0.3mm artificial feed after four
weeks we got fingerlings for stocking. FOR MORE INFORMATION OR POND CONSTRUTION
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