3.1 Research
Design: The study is an experimental, prospective and
descriptive research design.
3.2 area of study: The study
area is Nnewi metropolis in Anambra State, is a cosmopolitan town. It has
a population of 4,177,828 according to
2006 census (national bureau of
statistics; 2009)
3.3. Study
population:
The population is motorcyclists
aged 21-50 and apparently healthy
students of the
same age bracket that
serves as the control subjects.
3.4 sample size:
50 motorcycle riders, 20
control subjects of age and sex
matched who are apparently
healthy.
3.5 instrumetns for samples collection : 2mls syringers sna dneedles, dry cotton
woll, spirit swab, EDTA –containers, EDTA Container stand
3.6 method of samples collection: 2mls of blood was collected into EDTA
–Bottles through venepuncture. They
were all analyzed immediately after collection, but where immediate analysis was not possible the
sample were stored at
4oC for 24 hours
3.7 statistical analysis: for statistical analysis of the samples the
mean, standard deviation, and
analysis of variance (ANOVA) was used.
3.8 Materials used:
1.
diluted
cell suspension
2.
diluents
(drabkin’s solution, trisodium citrate, ammonium oxalate).
3.
Sysmex machine
4.
Clean
grease –free slides
5.
Spreader
6.
Cotton
wool.
7.
EDTA
–anti –coagulated blood
8.
Leishmann
stain
9.
Methanol
10. Buffered water
11. Erythrocyte
sedimentation rate (ESR) TUBES AND STANDS.
12. Pipette filler
13. Stop watch
14. Capillary tubes
15. Micro-haematocrit
reader and centrifuge
16. Spectrophotometer
17. Microscope
18. Improved
neubauer conting chamber (INCC)
19. Counting chamber cover galss
20. A safe
filling device
21. Hand counter