Method
Used:
Haemiglobincyanide (HiCN) Method.
Principle: whole blood is diluted 1 in 201
dilutions in a modified
Drabkin’s solution which contains
potassium cyanide. The red cells are haemolyzed and the Hb is
oxidized by the ferricyanide to methaemoglobin. This is converted by the
cyanide to stable hemoglobin cyanide
(HiCN). Absorbance of the HiCN solution is read in a
spectrophotometer at wavelength 540nm or in a filter Colorimeter using a
yellow-green filter (Cheesbrough, 2000) .
Procedure:
1.
measured 20µ1 (0.02ml)
of capillary blood on well –mixed venous blood and
dispense it into 4ml Drabkin’s
neutal diluting fluid
2.
stoppered
the tube, mixed and left the diluted blood at room
temperature protected from sunlight for 5-5minutes
3.
placed
the yellow –green filter in a colorimeter or set at a wavelength at 540nm
4.
zero the colorimeter with Drabkins fluid and read
the absorbance of the patients sample
(CHEESBROUGHT, 2000)